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UniBond League
Premier Division
Season 1999-2000
| | P | W | D | L | F-A | Pts |
| 1 | Leigh RMI | 44 | 28 | 8 | 8 | 91-45 | 92 |
| 2 | Hyde United | 44 | 24 | 13 | 7 | 77-44 | 85 |
| 3 | Gateshead | 44 | 23 | 13 | 8 | 79-41 | 82 |
| 4 | Marine | 44 | 21 | 16 | 7 | 78-46 | 79 |
| 5 | Emley | 44 | 20 | 12 | 12 | 54-41 | 72 |
| 6 | Lancaster City | 44 | 20 | 11 | 13 | 65-55 | 71 |
| 7 | Stalybridge Celtic | 44 | 18 | 12 | 14 | 64-54 | 66 |
| 8 | Bishop Auckland | 44 | 18 | 11 | 15 | 63-61 | 65 |
| 9 | Runcorn | 44 | 18 | 10 | 16 | 64-55 | 64 |
| 10 | Worksop Town | 44 | 19 | 6 | 19 | 78-65 | 63 |
| 11 | Gainsborough Trinity | 44 | 16 | 15 | 13 | 59-49 | 63 |
| 12 | Whitby Town | 44 | 15 | 13 | 16 | 66-66 | 58 |
| 13 | Barrow | 44 | 14 | 15 | 15 | 65-59 | 57 |
| 14 | Blyth Spartans | 44 | 15 | 9 | 20 | 62-67 | 54 |
| 15 | Droylsden | 44 | 14 | 12 | 18 | 53-60 | 54 |
| 16 | Frickley Athletic | 44 | 15 | 9 | 20 | 64-85 | 54 |
| 17 | Bamber Bridge | 44 | 14 | 11 | 19 | 70-67 | 53 |
| 18 | Hucknall Town | 44 | 14 | 11 | 19 | 55-61 | 53 |
| 19 | Leek Town | 44 | 14 | 10 | 20 | 58-79 | 52 |
| 20 | Colwyn Bay | 44 | 12 | 12 | 20 | 46-85 | 48 |
| 21 | Spennymoor United | 44 | 10 | 13 | 21 | 41-71 | 42 |
| 22 | Guiseley | 44 | 8 | 17 | 19 | 52-72 | 41 |
| 23 | Winsford United | 44 | 3 | 7 | 34 | 40-116 | 16 |
Spennymoor United: 1 point deducted
Leigh RMI promoted to the Nationwide Conference
Altrincham relegated from the Nationwide Conference
Accrington Stanley and Burscough promoted from Division One
Guiseley and Winsford United relegated to Division One
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