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UniBond League
Premier Division
Season 2000-01
| Saturday 19th August 2000 |
| Altrincham | 3-2 | Gateshead |
| Barrow | 1-3 | Emley |
| Blyth Spartans | 1-0 | Gainsborough Trinity |
| Burscough | 1-0 | Hyde United |
| Colwyn Bay | 3-3 | Bishop Auckland |
| Droylsden | 1-3 | Whitby Town |
| Frickley Athletic | 1-2 | Bamber Bridge |
| Leek Town | 0-2 | Accrington Stanley |
| Marine | 1-1 | Hucknall Town |
| Stalybridge Celtic | 3-2 | Spennymoor United |
| Worksop Town | 1-1 | Runcorn |
| Monday 21st August 2000 |
| Hyde United | 2-1 | Leek Town |
| Tuesday 22nd August 2000 |
| Accrington Stanley | 2-1 | Altrincham |
| Bamber Bridge | 1-2 | Barrow |
| Blyth Spartans | 1-2 | Bishop Auckland |
| Droylsden | 1-0 | Colwyn Bay |
| Gainsborough Trinity | 2-2 | Frickley Athletic |
| Hucknall Town | 1-1 | Worksop Town |
| Lancaster City | 5-2 | Burscough |
| Runcorn | 2-2 | Marine |
| Spennymoor United | 1-2 | Whitby Town |
| Saturday 26th August 2000 |
| Accrington Stanley | 3-1 | Emley |
| Bamber Bridge | 3-4 | Colwyn Bay |
| Gainsborough Trinity | 2-1 | Burscough |
| Hucknall Town | 3-1 | Frickley Athletic |
| Hyde United | 6-2 | Blyth Spartans |
| Lancaster City | 2-0 | Gateshead |
| Marine | 0-3 | Stalybridge Celtic |
| Runcorn | 0-1 | Leek Town |
| Spennymoor United | 2-3 | Barrow |
| Whitby Town | 2-2 | Altrincham |
| Worksop Town | 5-0 | Droylsden |
| Monday 28th August 2000 |
| Altrincham | 3-1 | Lancaster City |
| Barrow | 3-0 | Accrington Stanley |
| Blyth Spartans | 2-1 | Gateshead |
| Burscough | 3-0 | Bamber Bridge |
| Colwyn Bay | 2-2 | Runcorn |
| Droylsden | 2-4 | Hyde United |
| Emley | 3-3 | Spennymoor United |
| Frickley Athletic | 0-1 | Bishop Auckland |
| Leek Town | 0-0 | Hucknall Town |
| Stalybridge Celtic | 1-1 | Whitby Town |
| Worksop Town | 1-1 | Gainsborough Trinity |
| Saturday 2nd September 2000 |
| Bishop Auckland | 1-1 | Marine |
| Burscough | 3-1 | Blyth Spartans |
| Emley | 1-0 | Bamber Bridge |
| Gainsborough Trinity | 0-1 | Droylsden |
| Gateshead | 2-2 | Stalybridge Celtic |
| Hyde United | 5-1 | Hucknall Town |
| Lancaster City | 2-0 | Worksop Town |
| Leek Town | 2-1 | Barrow |
| Runcorn | 2-1 | Frickley Athletic |
| Spennymoor United | 1-1 | Colwyn Bay |
| Whitby Town | 0-0 | Accrington Stanley |
| Tuesday 5th September 2000 |
| Accrington Stanley | 2-1 | Runcorn |
| Bamber Bridge | 1-4 | Lancaster City |
| Barrow | 2-2 | Burscough |
| Colwyn Bay | 2-1 | Hyde United |
| Hucknall Town | 1-0 | Gainsborough Trinity |
| Marine | 0-4 | Altrincham |
| Stalybridge Celtic | 4-1 | Droylsden |
| Whitby Town | 1-0 | Blyth Spartans |
| Worksop Town | 3-1 | Leek Town |
| Wednesday 6th September 2000 |
| Bishop Auckland | 1-3 | Emley |
| Gateshead | 4-0 | Spennymoor United |
| Saturday 9th September 2000 |
| Accrington Stanley | 3-4 | Worksop Town |
| Altrincham | 0-2 | Emley |
| Bamber Bridge | 2-0 | Gainsborough Trinity |
| Barrow | 1-0 | Hyde United |
| Blyth Spartans | 0-0 | Leek Town |
| Colwyn Bay | 2-2 | Gateshead |
| Droylsden | 4-1 | Spennymoor United |
| Frickley Athletic | 1-0 | Burscough |
| Hucknall Town | 3-1 | Lancaster City |
| Marine | 4-1 | Whitby Town |
| Stalybridge Celtic | 2-0 | Bishop Auckland |
| Monday 11th September 2000 |
| Emley | 0-0 | Hucknall Town |
| Hyde United | 1-3 | Worksop Town |
| Tuesday 12th September 2000 |
| Altrincham | 3-0 | Colwyn Bay |
| Burscough | 1-3 | Marine |
| Gainsborough Trinity | 0-0 | Stalybridge Celtic |
| Lancaster City | 1-1 | Accrington Stanley |
| Runcorn | 2-3 | Bamber Bridge |
| Spennymoor United | 1-2 | Blyth Spartans |
| Wednesday 13th September 2000 |
| Bishop Auckland | 2-0 | Frickley Athletic |
| Saturday 16th September 2000 |
| Bishop Auckland | 3-1 | Gainsborough Trinity |
| Blyth Spartans | 0-2 | Whitby Town |
| Burscough | 4-1 | Spennymoor United |
| Emley | 1-0 | Lancaster City |
| Gateshead | 5-1 | Runcorn |
| Hucknall Town | 2-0 | Accrington Stanley |
| Hyde United | 0-0 | Frickley Athletic |
| Leek Town | 2-2 | Marine |
| Worksop Town | 1-2 | Altrincham |
| Tuesday 19th September 2000 |
| Accrington Stanley | 3-1 | Bishop Auckland |
| Colwyn Bay | 4-1 | Burscough |
| Frickley Athletic | 1-5 | Worksop Town |
| Gainsborough Trinity | 3-2 | Emley |
| Marine | 1-1 | Lancaster City |
| Spennymoor United | 1-4 | Gateshead |
| Saturday 23rd September 2000 |
| Accrington Stanley | 1-4 | Stalybridge Celtic |
| Colwyn Bay | 1-0 | Blyth Spartans |
| Frickley Athletic | 0-1 | Marine |
| Gainsborough Trinity | 0-2 | Gateshead |
| Hyde United | 1-1 | Bishop Auckland |
| Lancaster City | 0-2 | Droylsden |
| Leek Town | 0-1 | Emley |
| Runcorn | 2-1 | Hucknall Town |
| Spennymoor United | 0-4 | Altrincham |
| Whitby Town | 0-2 | Burscough |
| Worksop Town | 4-2 | Barrow |
| Monday 25th September 2000 |
| Emley | 3-2 | Marine |
| Hyde United | 1-1 | Colwyn Bay |
| Tuesday 26th September 2000 |
| Burscough | 0-0 | Accrington Stanley |
| Droylsden | 4-0 | Gainsborough Trinity |
| Hucknall Town | 2-2 | Stalybridge Celtic |
| Lancaster City | 3-2 | Bamber Bridge |
| Runcorn | 0-2 | Altrincham |
| Whitby Town | 3-3 | Spennymoor United |
| Worksop Town | 12-0 | Frickley Athletic |
| Wednesday 27th September 2000 |
| Bishop Auckland | 1-0 | Blyth Spartans |
| Gateshead | 1-2 | Barrow |
| Tuesday 3rd October 2000 |
| Altrincham | 0-0 | Stalybridge Celtic |
| Blyth Spartans | 0-1 | Spennymoor United |
| Colwyn Bay | 3-2 | Droylsden |
| Frickley Athletic | 1-2 | Hucknall Town |
| Whitby Town | 0-1 | Emley |
| Wednesday 4th October 2000 |
| Bishop Auckland | 0-1 | Gateshead |
| Friday 6th October 2000 |
| Barrow | 3-1 | Bamber Bridge |
| Bishop Auckland | 2-1 | Whitby Town |
| Emley | 5-3 | Worksop Town |
| Stalybridge Celtic | 5-0 | Lancaster City |
| Saturday 7th October 2000 |
| Gateshead | 5-0 | Frickley Athletic |
| Marine | 3-5 | Burscough |
| Sunday 8th October 2000 |
| Accrington Stanley | 2-1 | Leek Town |
| Blyth Spartans | 1-1 | Hucknall Town |
| Droylsden | 2-1 | Altrincham |
| Tuesday 10th October 2000 |
| Altrincham | 1-1 | Leek Town |
| Frickley Athletic | 2-2 | Gainsborough Trinity |
| Marine | 1-1 | Hyde United |
| Spennymoor United | 1-6 | Accrington Stanley |
| Worksop Town | 3-2 | Whitby Town |
| Saturday 14th October 2000 |
| Altrincham | 5-0 | Spennymoor United |
| Blyth Spartans | 0-1 | Droylsden |
| Colwyn Bay | 3-2 | Worksop Town |
| Lancaster City | 3-1 | Runcorn |
| Monday 16th October 2000 |
| Emley | 1-0 | Blyth Spartans |
| Hyde United | 4-2 | Altrincham |
| Tuesday 17th October 2000 |
| Lancaster City | 3-1 | Marine |
| Wednesday 18th October 2000 |
| Bishop Auckland | 2-1 | Worksop Town |
| Saturday 21st October 2000 |
| Altrincham | 3-1 | Blyth Spartans |
| Bamber Bridge | 1-0 | Leek Town |
| Burscough | 2-1 | Bishop Auckland |
| Emley | 2-1 | Droylsden |
| Frickley Athletic | 0-2 | Accrington Stanley |
| Hucknall Town | 1-4 | Barrow |
| Marine | 1-1 | Gateshead |
| Spennymoor United | 3-0 | Hyde United |
| Stalybridge Celtic | 7-1 | Gainsborough Trinity |
| Whitby Town | 3-1 | Runcorn |
| Worksop Town | 4-2 | Lancaster City |
| Tuesday 24th October 2000 |
| Accrington Stanley | 1-2 | Lancaster City |
| Bamber Bridge | 2-1 | Droylsden |
| Barrow | 2-3 | Altrincham |
| Gainsborough Trinity | 2-1 | Hyde United |
| Hucknall Town | 0-1 | Whitby Town |
| Marine | 4-1 | Runcorn |
| Stalybridge Celtic | 1-0 | Worksop Town |
| Wednesday 25th October 2000 |
| Gateshead | 0-1 | Emley |
| Saturday 28th October 2000 |
| Accrington Stanley | 1-0 | Spennymoor United |
| Bishop Auckland | 3-1 | Leek Town |
| Droylsden | 1-2 | Blyth Spartans |
| Gainsborough Trinity | 2-0 | Marine |
| Whitby Town | 1-1 | Bamber Bridge |
| Saturday 4th November 2000 |
| Emley | 2-0 | Gateshead |
| Hyde United | 2-1 | Marine |
| Saturday 11th November 2000 |
| Altrincham | 0-2 | Gainsborough Trinity |
| Barrow | 2-3 | Whitby Town |
| Blyth Spartans | 1-0 | Accrington Stanley |
| Droylsden | 0-3 | Bishop Auckland |
| Gateshead | 2-1 | Leek Town |
| Marine | 1-0 | Spennymoor United |
| Runcorn | 1-0 | Colwyn Bay |
| Stalybridge Celtic | 3-0 | Bamber Bridge |
| Saturday 18th November 2000 |
| Accrington Stanley | 3-0 | Droylsden |
| Bamber Bridge | 2-0 | Marine |
| Bishop Auckland | 2-0 | Altrincham |
| Burscough | 2-2 | Worksop Town |
| Colwyn Bay | 0-2 | Hucknall Town |
| Gainsborough Trinity | 2-0 | Runcorn |
| Hyde United | 3-2 | Whitby Town |
| Lancaster City | 1-1 | Blyth Spartans |
| Leek Town | 0-2 | Stalybridge Celtic |
| Spennymoor United | 0-2 | Emley |
| Saturday 25th November 2000 |
| Bamber Bridge | 1-1 | Altrincham |
| Frickley Athletic | 6-2 | Colwyn Bay |
| Gainsborough Trinity | 2-0 | Barrow |
| Marine | 1-3 | Bishop Auckland |
| Runcorn | 2-0 | Accrington Stanley |
| Spennymoor United | 0-2 | Burscough |
| Stalybridge Celtic | 4-2 | Emley |
| Whitby Town | 1-2 | Leek Town |
| Worksop Town | 4-3 | Blyth Spartans |
| Saturday 2nd December 2000 |
| Barrow | 2-0 | Droylsden |
| Colwyn Bay | 1-1 | Bamber Bridge |
| Leek Town | 0-1 | Whitby Town |
| Saturday 9th December 2000 |
| Accrington Stanley | 2-2 | Colwyn Bay |
| Altrincham | 3-1 | Marine |
| Burscough | 1-0 | Leek Town |
| Emley | 0-0 | Barrow |
| Gainsborough Trinity | 2-0 | Bamber Bridge |
| Hucknall Town | 1-0 | Spennymoor United |
| Lancaster City | 3-2 | Stalybridge Celtic |
| Saturday 16th December 2000 |
| Bamber Bridge | 1-3 | Hucknall Town |
| Barrow | 1-4 | Bishop Auckland |
| Frickley Athletic | 1-1 | Droylsden |
| Hyde United | 3-3 | Accrington Stanley |
| Leek Town | 0-0 | Altrincham |
| Marine | 0-2 | Emley |
| Runcorn | 1-0 | Blyth Spartans |
| Whitby Town | 0-1 | Stalybridge Celtic |
| Saturday 23rd December 2000 |
| Altrincham | 3-1 | Whitby Town |
| Bishop Auckland | 5-2 | Bamber Bridge |
| Blyth Spartans | 3-0 | Runcorn |
| Burscough | 1-2 | Barrow |
| Droylsden | 0-0 | Gateshead |
| Emley | 1-0 | Accrington Stanley |
| Hucknall Town | 1-0 | Colwyn Bay |
| Lancaster City | 2-1 | Gainsborough Trinity |
| Leek Town | 3-1 | Spennymoor United |
| Marine | 0-1 | Worksop Town |
| Stalybridge Celtic | 3-0 | Frickley Athletic |
| Tuesday 26th December 2000 |
| Accrington Stanley | 1-3 | Bamber Bridge |
| Colwyn Bay | 2-3 | Marine |
| Frickley Athletic | 1-3 | Emley |
| Gainsborough Trinity | 1-1 | Worksop Town |
| Gateshead | 2-5 | Blyth Spartans |
| Hyde United | 1-2 | Stalybridge Celtic |
| Lancaster City | 1-1 | Barrow |
| Runcorn | 2-0 | Burscough |
| Whitby Town | 0-1 | Droylsden |
| Monday 1st January 2001 |
| Altrincham | 3-1 | Frickley Athletic |
| Barrow | 2-2 | Lancaster City |
| Emley | 1-0 | Gainsborough Trinity |
| Saturday 6th January 2001 |
| Altrincham | 2-2 | Burscough |
| Bishop Auckland | 2-1 | Accrington Stanley |
| Frickley Athletic | 1-1 | Hyde United |
| Gainsborough Trinity | 1-0 | Colwyn Bay |
| Gateshead | 0-2 | Bamber Bridge |
| Lancaster City | 3-3 | Emley |
| Leek Town | 0-4 | Blyth Spartans |
| Marine | 1-0 | Droylsden |
| Runcorn | 2-0 | Spennymoor United |
| Stalybridge Celtic | 1-0 | Barrow |
| Whitby Town | 1-1 | Worksop Town |
| Tuesday 9th January 2001 |
| Stalybridge Celtic | 3-1 | Runcorn |
| Saturday 13th January 2001 |
| Bamber Bridge | 0-1 | Frickley Athletic |
| Droylsden | 2-1 | Barrow |
| Gateshead | 2-1 | Altrincham |
| Lancaster City | 2-0 | Spennymoor United |
| Leek Town | 1-0 | Gainsborough Trinity |
| Whitby Town | 5-1 | Colwyn Bay |
| Saturday 20th January 2001 |
| Accrington Stanley | 1-1 | Gainsborough Trinity |
| Barrow | 1-2 | Runcorn |
| Blyth Spartans | 1-1 | Altrincham |
| Emley | 4-0 | Whitby Town |
| Saturday 27th January 2001 |
| Altrincham | 1-2 | Worksop Town |
| Barrow | 1-0 | Marine |
| Bishop Auckland | 4-0 | Burscough |
| Blyth Spartans | 0-0 | Bamber Bridge |
| Colwyn Bay | 0-4 | Emley |
| Frickley Athletic | 1-1 | Runcorn |
| Gainsborough Trinity | 1-3 | Whitby Town |
| Hucknall Town | 1-2 | Hyde United |
| Lancaster City | 3-2 | Leek Town |
| Spennymoor United | 0-1 | Droylsden |
| Stalybridge Celtic | 2-1 | Gateshead |
| Saturday 3rd February 2001 |
| Bishop Auckland | 2-2 | Barrow |
| Burscough | 2-1 | Hucknall Town |
| Frickley Athletic | 2-0 | Spennymoor United |
| Gainsborough Trinity | 1-1 | Lancaster City |
| Gateshead | 4-2 | Colwyn Bay |
| Leek Town | 1-1 | Bamber Bridge |
| Runcorn | 3-1 | Droylsden |
| Stalybridge Celtic | 4-0 | Accrington Stanley |
| Tuesday 6th February 2001 |
| Accrington Stanley | 3-1 | Gateshead |
| Droylsden | 1-1 | Frickley Athletic |
| Hucknall Town | 0-2 | Emley |
| Saturday 10th February 2001 |
| Accrington Stanley | 1-1 | Frickley Athletic |
| Bamber Bridge | 5-0 | Whitby Town |
| Burscough | 1-1 | Gainsborough Trinity |
| Droylsden | 1-1 | Hucknall Town |
| Emley | 1-1 | Leek Town |
| Hyde United | 3-1 | Gateshead |
| Lancaster City | 4-1 | Altrincham |
| Worksop Town | 3-3 | Marine |
| Tuesday 13th February 2001 |
| Blyth Spartans | 1-1 | Barrow |
| Frickley Athletic | 3-3 | Altrincham |
| Stalybridge Celtic | 3-1 | Hyde United |
| Saturday 17th February 2001 |
| Accrington Stanley | 4-1 | Hyde United |
| Altrincham | 0-2 | Bishop Auckland |
| Bamber Bridge | 1-1 | Emley |
| Barrow | 2-0 | Blyth Spartans |
| Droylsden | 2-5 | Worksop Town |
| Frickley Athletic | 1-3 | Leek Town |
| Gateshead | 3-0 | Marine |
| Hucknall Town | 3-2 | Burscough |
| Runcorn | 1-1 | Gainsborough Trinity |
| Whitby Town | 1-5 | Lancaster City |
| Monday 19th February 2001 |
| Hyde United | 4-4 | Barrow |
| Tuesday 20th February 2001 |
| Altrincham | 1-0 | Hucknall Town |
| Burscough | 1-1 | Stalybridge Celtic |
| Colwyn Bay | 2-4 | Accrington Stanley |
| Leek Town | 2-1 | Runcorn |
| Spennymoor United | 0-5 | Frickley Athletic |
| Wednesday 21st February 2001 |
| Bishop Auckland | 1-3 | Lancaster City |
| Saturday 24th February 2001 |
| Bishop Auckland | 2-1 | Droylsden |
| Burscough | 2-3 | Whitby Town |
| Emley | 4-1 | Colwyn Bay |
| Gainsborough Trinity | 2-0 | Accrington Stanley |
| Hucknall Town | 1-0 | Bamber Bridge |
| Lancaster City | 1-4 | Hyde United |
| Leek Town | 1-2 | Gateshead |
| Marine | 1-0 | Frickley Athletic |
| Runcorn | 3-1 | Barrow |
| Stalybridge Celtic | 1-0 | Altrincham |
| Tuesday 27th February 2001 |
| Bamber Bridge | 2-1 | Hyde United |
| Barrow | 0-0 | Stalybridge Celtic |
| Frickley Athletic | 4-2 | Blyth Spartans |
| Hucknall Town | 2-0 | Leek Town |
| Saturday 3rd March 2001 |
| Colwyn Bay | 0-0 | Frickley Athletic |
| Tuesday 6th March 2001 |
| Altrincham | 1-2 | Bamber Bridge |
| Droylsden | 0-2 | Runcorn |
| Emley | 3-1 | Bishop Auckland |
| Gainsborough Trinity | 2-1 | Spennymoor United |
| Leek Town | 2-0 | Hyde United |
| Stalybridge Celtic | 1-0 | Hucknall Town |
| Worksop Town | 2-2 | Gateshead |
| Saturday 10th March 2001 |
| Barrow | 3-0 | Leek Town |
| Blyth Spartans | 5-2 | Colwyn Bay |
| Droylsden | 1-2 | Burscough |
| Frickley Athletic | 0-0 | Stalybridge Celtic |
| Gateshead | 0-0 | Accrington Stanley |
| Hucknall Town | 1-1 | Marine |
| Hyde United | 0-1 | Gainsborough Trinity |
| Lancaster City | 3-1 | Whitby Town |
| Runcorn | 4-4 | Bishop Auckland |
| Spennymoor United | 1-2 | Bamber Bridge |
| Tuesday 13th March 2001 |
| Barrow | 3-2 | Colwyn Bay |
| Burscough | 0-1 | Emley |
| Gainsborough Trinity | 1-1 | Hucknall Town |
| Spennymoor United | 0-3 | Lancaster City |
| Whitby Town | 2-2 | Frickley Athletic |
| Worksop Town | 1-1 | Stalybridge Celtic |
| Wednesday 14th March 2001 |
| Gateshead | 0-3 | Bishop Auckland |
| Friday 16th March 2001 |
| Runcorn | 0-4 | Worksop Town |
| Saturday 17th March 2001 |
| Accrington Stanley | 0-2 | Whitby Town |
| Altrincham | 3-0 | Droylsden |
| Bamber Bridge | 1-3 | Blyth Spartans |
| Barrow | 5-0 | Hucknall Town |
| Bishop Auckland | 2-0 | Spennymoor United |
| Burscough | 0-1 | Gateshead |
| Colwyn Bay | 1-2 | Lancaster City |
| Emley | 3-1 | Hyde United |
| Leek Town | 2-1 | Frickley Athletic |
| Stalybridge Celtic | 2-1 | Marine |
| Tuesday 20th March 2001 |
| Blyth Spartans | 3-0 | Frickley Athletic |
| Colwyn Bay | 1-7 | Stalybridge Celtic |
| Marine | 1-1 | Accrington Stanley |
| Spennymoor United | 0-3 | Gainsborough Trinity |
| Worksop Town | 1-0 | Burscough |
| Wednesday 21st March 2001 |
| Bishop Auckland | 0-2 | Runcorn |
| Gateshead | 1-1 | Whitby Town |
| Friday 23rd March 2001 |
| Bamber Bridge | 0-2 | Stalybridge Celtic |
| Lancaster City | 1-0 | Bishop Auckland |
| Whitby Town | 0-1 | Gainsborough Trinity |
| Worksop Town | 1-2 | Emley |
| Saturday 24th March 2001 |
| Burscough | 0-0 | Runcorn |
| Frickley Athletic | 1-0 | Gateshead |
| Hyde United | 4-0 | Spennymoor United |
| Marine | 2-0 | Colwyn Bay |
| Sunday 25th March 2001 |
| Altrincham | 5-2 | Accrington Stanley |
| Droylsden | 1-0 | Leek Town |
| Hucknall Town | 4-1 | Blyth Spartans |
| Whitby Town | 1-3 | Hyde United |
| Tuesday 27th March 2001 |
| Accrington Stanley | 1-2 | Hucknall Town |
| Altrincham | 1-1 | Barrow |
| Bamber Bridge | 0-2 | Bishop Auckland |
| Blyth Spartans | 1-0 | Worksop Town |
| Colwyn Bay | 3-2 | Spennymoor United |
| Droylsden | 3-2 | Lancaster City |
| Stalybridge Celtic | 1-2 | Leek Town |
| Wednesday 28th March 2001 |
| Gateshead | 1-1 | Burscough |
| Runcorn | 1-1 | Hyde United |
| Friday 30th March 2001 |
| Hyde United | 1-2 | Emley |
| Saturday 31st March 2001 |
| Barrow | 2-0 | Frickley Athletic |
| Bishop Auckland | 5-1 | Colwyn Bay |
| Blyth Spartans | 0-1 | Lancaster City |
| Gainsborough Trinity | 1-0 | Altrincham |
| Gateshead | 1-1 | Droylsden |
| Hucknall Town | 1-0 | Runcorn |
| Leek Town | 4-0 | Burscough |
| Spennymoor United | 0-1 | Stalybridge Celtic |
| Whitby Town | 3-2 | Marine |
| Worksop Town | 1-1 | Bamber Bridge |
| Monday 2nd April 2001 |
| Emley | 2-1 | Burscough |
| Tuesday 3rd April 2001 |
| Bamber Bridge | 4-2 | Gateshead |
| Colwyn Bay | 1-2 | Altrincham |
| Hucknall Town | 1-2 | Droylsden |
| Saturday 7th April 2001 |
| Colwyn Bay | 3-3 | Gainsborough Trinity |
| Emley | 3-1 | Altrincham |
| Runcorn | 2-1 | Whitby Town |
| Tuesday 10th April 2001 |
| Blyth Spartans | 1-2 | Stalybridge Celtic |
| Gainsborough Trinity | 4-1 | Leek Town |
| Hucknall Town | 2-5 | Bishop Auckland |
| Lancaster City | 1-0 | Colwyn Bay |
| Marine | 2-1 | Bamber Bridge |
| Whitby Town | 0-2 | Barrow |
| Worksop Town | 6-0 | Spennymoor United |
| Thursday 12th April 2001 |
| Barrow | 3-1 | Gateshead |
| Droylsden | 2-3 | Marine |
| Hyde United | 1-1 | Runcorn |
| Lancaster City | 4-1 | Hucknall Town |
| Leek Town | 0-3 | Worksop Town |
| Spennymoor United | 0-3 | Bishop Auckland |
| Saturday 14th April 2001 |
| Accrington Stanley | 3-2 | Marine |
| Altrincham | 2-1 | Runcorn |
| Barrow | 5-0 | Spennymoor United |
| Bishop Auckland | 2-0 | Hucknall Town |
| Blyth Spartans | 2-1 | Emley |
| Burscough | 2-3 | Lancaster City |
| Droylsden | 0-1 | Bamber Bridge |
| Frickley Athletic | 1-1 | Whitby Town |
| Gateshead | 1-1 | Gainsborough Trinity |
| Stalybridge Celtic | 4-0 | Colwyn Bay |
| Worksop Town | 4-1 | Hyde United |
| Monday 16th April 2001 |
| Bamber Bridge | 3-0 | Accrington Stanley |
| Burscough | 1-1 | Altrincham |
| Emley | 0-0 | Frickley Athletic |
| Gainsborough Trinity | 4-2 | Blyth Spartans |
| Hucknall Town | 2-2 | Gateshead |
| Hyde United | 3-2 | Droylsden |
| Leek Town | 2-4 | Colwyn Bay |
| Marine | 1-2 | Barrow |
| Runcorn | 2-1 | Stalybridge Celtic |
| Spennymoor United | 0-1 | Worksop Town |
| Whitby Town | 1-1 | Bishop Auckland |
| Wednesday 18th April 2001 |
| Bishop Auckland | 3-1 | Hyde United |
| Burscough | 1-2 | Frickley Athletic |
| Leek Town | 0-2 | Lancaster City |
| Stalybridge Celtic | 2-0 | Blyth Spartans |
| Worksop Town | 1-1 | Hucknall Town |
| Saturday 21st April 2001 |
| Barrow | 3-2 | Gainsborough Trinity |
| Bishop Auckland | 0-2 | Stalybridge Celtic |
| Blyth Spartans | 1-0 | Burscough |
| Colwyn Bay | 2-1 | Whitby Town |
| Droylsden | 0-4 | Accrington Stanley |
| Frickley Athletic | 0-0 | Lancaster City |
| Gateshead | 0-0 | Worksop Town |
| Hyde United | 1-4 | Bamber Bridge |
| Marine | 2-0 | Leek Town |
| Runcorn | 1-1 | Emley |
| Spennymoor United | 0-1 | Hucknall Town |
| Tuesday 24th April 2001 |
| Accrington Stanley | 2-1 | Barrow |
| Bamber Bridge | 0-1 | Burscough |
| Emley | 2-1 | Runcorn |
| Whitby Town | 0-1 | Gateshead |
| Worksop Town | 1-2 | Bishop Auckland |
| Thursday 26th April 2001 |
| Blyth Spartans | 4-1 | Marine |
| Burscough | 1-2 | Colwyn Bay |
| Gateshead | 3-0 | Hyde United |
| Runcorn | 1-0 | Lancaster City |
| Spennymoor United | 2-0 | Leek Town |
| Saturday 28th April 2001 |
| Accrington Stanley | 3-3 | Blyth Spartans |
| Altrincham | 2-0 | Hyde United |
| Bamber Bridge | 3-1 | Runcorn |
| Barrow | 2-1 | Worksop Town |
| Burscough | 3-1 | Droylsden |
| Colwyn Bay | 4-1 | Leek Town |
| Emley | 2-3 | Stalybridge Celtic |
| Gainsborough Trinity | 0-0 | Bishop Auckland |
| Gateshead | 1-0 | Lancaster City |
| Spennymoor United | 2-2 | Marine |
| Whitby Town | 2-2 | Hucknall Town |
| Monday 30th April 2001 |
| Hyde United | 1-1 | Burscough |
| Tuesday 1st May 2001 |
| Droylsden | 0-1 | Stalybridge Celtic |
| Frickley Athletic | 3-2 | Barrow |
| Marine | 1-1 | Blyth Spartans |
| Spennymoor United | 2-0 | Runcorn |
| Worksop Town | 1-1 | Colwyn Bay |
| Wednesday 2nd May 2001 |
| Accrington Stanley | 2-0 | Burscough |
| Gateshead | 1-2 | Hucknall Town |
| Thursday 3rd May 2001 |
| Bamber Bridge | 1-2 | Worksop Town |
| Hyde United | 1-1 | Lancaster City |
| Leek Town | 1-1 | Droylsden |
| Saturday 5th May 2001 |
| Bamber Bridge | 0-0 | Spennymoor United |
| Blyth Spartans | 0-0 | Hyde United |
| Colwyn Bay | 2-0 | Barrow |
| Droylsden | 1-3 | Emley |
| Hucknall Town | 1-3 | Altrincham |
| Lancaster City | 0-1 | Frickley Athletic |
| Leek Town | 3-1 | Bishop Auckland |
| Marine | 2-2 | Gainsborough Trinity |
| Runcorn | 1-2 | Gateshead |
| Stalybridge Celtic | 0-2 | Burscough |
| Worksop Town | 0-2 | Accrington Stanley |
|
