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Dr Martens League
Premier Division
Season 2000-01
| Saturday 19th August 2000 |
| Bath City | 2-1 | Folkestone Invicta |
| Cambridge City | 1-0 | Havant & Waterlooville |
| Clevedon Town | 2-3 | Welling United |
| Dorchester Town | 2-2 | Fisher Athletic |
| King's Lynn | 2-1 | Weymouth |
| Margate | 0-1 | Moor Green |
| Merthyr Tydfil | 4-4 | Ilkeston Town |
| Newport County | 1-1 | Burton Albion |
| Salisbury City | 2-1 | Halesowen Town |
| Stafford Rangers | 1-2 | Crawley Town |
| Tamworth | 1-2 | Worcester City |
| Monday 21st August 2000 |
| Ilkeston Town | 2-1 | Tamworth |
| Worcester City | 0-0 | Bath City |
| Tuesday 22nd August 2000 |
| Burton Albion | 1-1 | King's Lynn |
| Clevedon Town | 3-1 | Salisbury City |
| Crawley Town | 1-0 | Dorchester Town |
| Folkestone Invicta | 4-3 | Cambridge City |
| Halesowen Town | 0-1 | Stafford Rangers |
| Havant & Waterlooville | 3-2 | Fisher Athletic |
| Moor Green | 1-1 | Newport County |
| Welling United | 1-0 | Margate |
| Weymouth | 1-1 | Merthyr Tydfil |
| Saturday 26th August 2000 |
| Burton Albion | 3-0 | Fisher Athletic |
| Crawley Town | 3-0 | King's Lynn |
| Folkestone Invicta | 2-1 | Salisbury City |
| Halesowen Town | 0-0 | Margate |
| Havant & Waterlooville | 3-2 | Tamworth |
| Ilkeston Town | 0-2 | Cambridge City |
| Merthyr Tydfil | 3-3 | Bath City |
| Moor Green | 3-3 | Clevedon Town |
| Welling United | 1-1 | Dorchester Town |
| Weymouth | 0-0 | Newport County |
| Worcester City | 2-4 | Stafford Rangers |
| Monday 28th August 2000 |
| Bath City | 3-2 | Havant & Waterlooville |
| Cambridge City | 1-0 | Crawley Town |
| Dorchester Town | 1-0 | Clevedon Town |
| Folkestone Invicta | 0-1 | Welling United |
| Halesowen Town | 1-3 | Newport County |
| King's Lynn | 0-1 | Moor Green |
| Margate | 5-0 | Fisher Athletic |
| Merthyr Tydfil | 4-1 | Worcester City |
| Salisbury City | 3-2 | Weymouth |
| Stafford Rangers | 4-2 | Ilkeston Town |
| Tamworth | 2-3 | Burton Albion |
| Saturday 2nd September 2000 |
| Burton Albion | 1-0 | Merthyr Tydfil |
| Cambridge City | 2-0 | Bath City |
| Clevedon Town | 1-2 | Crawley Town |
| Fisher Athletic | 1-2 | Salisbury City |
| Havant & Waterlooville | 4-1 | King's Lynn |
| Ilkeston Town | 2-4 | Worcester City |
| Moor Green | 1-2 | Folkestone Invicta |
| Newport County | 5-0 | Dorchester Town |
| Stafford Rangers | 0-2 | Margate |
| Welling United | 4-0 | Tamworth |
| Weymouth | 4-1 | Halesowen Town |
| Monday 4th September 2000 |
| Ilkeston Town | 1-0 | Moor Green |
| Worcester City | 3-3 | Clevedon Town |
| Tuesday 5th September 2000 |
| Crawley Town | 4-1 | Folkestone Invicta |
| Dorchester Town | 4-3 | Bath City |
| Fisher Athletic | 1-2 | Weymouth |
| Halesowen Town | 1-2 | Burton Albion |
| Havant & Waterlooville | 0-1 | Welling United |
| Margate | 2-1 | Cambridge City |
| Merthyr Tydfil | 3-0 | Stafford Rangers |
| Salisbury City | 4-0 | Newport County |
| Tamworth | 2-0 | King's Lynn |
| Saturday 9th September 2000 |
| Bath City | 4-3 | Fisher Athletic |
| Dorchester Town | 1-2 | Ilkeston Town |
| Folkestone Invicta | 0-4 | Havant & Waterlooville |
| Halesowen Town | 2-2 | Crawley Town |
| King's Lynn | 3-1 | Clevedon Town |
| Margate | 1-3 | Burton Albion |
| Salisbury City | 2-2 | Merthyr Tydfil |
| Stafford Rangers | 1-1 | Newport County |
| Tamworth | 0-0 | Cambridge City |
| Welling United | 2-0 | Weymouth |
| Worcester City | 1-0 | Moor Green |
| Saturday 16th September 2000 |
| Burton Albion | 1-1 | Welling United |
| Cambridge City | 2-3 | Worcester City |
| Clevedon Town | 1-4 | Halesowen Town |
| Crawley Town | 5-1 | Salisbury City |
| Fisher Athletic | 2-3 | King's Lynn |
| Havant & Waterlooville | 2-3 | Margate |
| Ilkeston Town | 3-0 | Bath City |
| Merthyr Tydfil | 1-2 | Dorchester Town |
| Moor Green | 1-2 | Stafford Rangers |
| Newport County | 0-1 | Tamworth |
| Weymouth | 1-1 | Folkestone Invicta |
| Monday 18th September 2000 |
| Cambridge City | 2-0 | Folkestone Invicta |
| Tuesday 19th September 2000 |
| Bath City | 3-2 | Worcester City |
| Dorchester Town | 2-2 | Crawley Town |
| King's Lynn | 1-1 | Burton Albion |
| Margate | 5-0 | Welling United |
| Merthyr Tydfil | 0-1 | Weymouth |
| Salisbury City | 4-1 | Clevedon Town |
| Stafford Rangers | 0-1 | Halesowen Town |
| Tamworth | 3-0 | Ilkeston Town |
| Wednesday 20th September 2000 |
| Newport County | 0-1 | Moor Green |
| Saturday 23rd September 2000 |
| Bath City | 1-1 | Margate |
| Burton Albion | 1-1 | Dorchester Town |
| Clevedon Town | 1-1 | Tamworth |
| Crawley Town | 3-1 | Merthyr Tydfil |
| Folkestone Invicta | 3-1 | Newport County |
| Halesowen Town | 0-2 | Fisher Athletic |
| King's Lynn | 3-2 | Stafford Rangers |
| Moor Green | 4-1 | Havant & Waterlooville |
| Welling United | 1-2 | Ilkeston Town |
| Weymouth | 1-1 | Cambridge City |
| Worcester City | 0-1 | Salisbury City |
| Friday 6th October 2000 |
| Cambridge City | 1-1 | Burton Albion |
| Ilkeston Town | 1-0 | Halesowen Town |
| Saturday 7th October 2000 |
| Havant & Waterlooville | 3-0 | Newport County |
| Margate | 3-0 | Weymouth |
| Salisbury City | 4-4 | Bath City |
| Stafford Rangers | 4-1 | Clevedon Town |
| Welling United | 1-4 | King's Lynn |
| Sunday 8th October 2000 |
| Dorchester Town | 0-1 | Worcester City |
| Fisher Athletic | 3-2 | Crawley Town |
| Merthyr Tydfil | 2-0 | Moor Green |
| Tamworth | 1-0 | Folkestone Invicta |
| Saturday 14th October 2000 |
| Moor Green | 1-0 | Crawley Town |
| Newport County | 0-2 | Halesowen Town |
| Saturday 21st October 2000 |
| Bath City | 2-2 | Stafford Rangers |
| Burton Albion | 5-0 | Salisbury City |
| Cambridge City | 0-1 | Merthyr Tydfil |
| Crawley Town | 2-1 | Welling United |
| Folkestone Invicta | 0-3 | Clevedon Town |
| Halesowen Town | 1-1 | Dorchester Town |
| King's Lynn | 2-3 | Newport County |
| Margate | 1-0 | Havant & Waterlooville |
| Moor Green | 3-1 | Fisher Athletic |
| Weymouth | 4-1 | Ilkeston Town |
| Worcester City | 1-0 | Tamworth |
| Monday 23rd October 2000 |
| Ilkeston Town | 1-1 | Stafford Rangers |
| Worcester City | 1-1 | Merthyr Tydfil |
| Tuesday 24th October 2000 |
| Burton Albion | 3-1 | Tamworth |
| Clevedon Town | 4-1 | Dorchester Town |
| Crawley Town | 1-2 | Cambridge City |
| Fisher Athletic | 1-0 | Margate |
| Moor Green | 2-1 | King's Lynn |
| Welling United | 1-1 | Folkestone Invicta |
| Saturday 28th October 2000 |
| Clevedon Town | 2-1 | Weymouth |
| King's Lynn | 0-0 | Folkestone Invicta |
| Tamworth | 0-2 | Crawley Town |
| Saturday 4th November 2000 |
| Bath City | 3-1 | Burton Albion |
| Margate | 3-1 | King's Lynn |
| Saturday 11th November 2000 |
| Burton Albion | 1-0 | Clevedon Town |
| Cambridge City | 5-6 | Newport County |
| Crawley Town | 2-2 | Stafford Rangers |
| Folkestone Invicta | 1-3 | Ilkeston Town |
| Halesowen Town | 1-0 | Tamworth |
| King's Lynn | 1-1 | Bath City |
| Merthyr Tydfil | 2-2 | Fisher Athletic |
| Salisbury City | 1-1 | Welling United |
| Weymouth | 1-2 | Havant & Waterlooville |
| Saturday 18th November 2000 |
| Bath City | 3-2 | Crawley Town |
| Clevedon Town | 1-2 | Moor Green |
| Fisher Athletic | 1-2 | Cambridge City |
| Halesowen Town | 4-4 | Folkestone Invicta |
| King's Lynn | 2-1 | Salisbury City |
| Margate | 3-2 | Merthyr Tydfil |
| Newport County | 1-1 | Welling United |
| Stafford Rangers | 1-2 | Worcester City |
| Tamworth | 2-3 | Weymouth |
| Tuesday 21st November 2000 |
| King's Lynn | 6-0 | Tamworth |
| Moor Green | 1-0 | Ilkeston Town |
| Saturday 25th November 2000 |
| Dorchester Town | 0-3 | Newport County |
| Folkestone Invicta | 1-2 | Moor Green |
| Halesowen Town | 1-1 | Clevedon Town |
| Margate | 3-1 | Stafford Rangers |
| Merthyr Tydfil | 2-2 | Cambridge City |
| Tamworth | 3-3 | Welling United |
| Worcester City | 0-0 | Weymouth |
| Saturday 2nd December 2000 |
| Salisbury City | 3-1 | Moor Green |
| Welling United | 2-2 | Halesowen Town |
| Saturday 9th December 2000 |
| Bath City | 3-1 | Moor Green |
| Burton Albion | 1-0 | Worcester City |
| Cambridge City | 1-2 | Salisbury City |
| Ilkeston Town | 1-1 | Clevedon Town |
| Merthyr Tydfil | 0-1 | Welling United |
| Newport County | 3-1 | Crawley Town |
| Stafford Rangers | 3-0 | Folkestone Invicta |
| Tamworth | 1-0 | Margate |
| Weymouth | 4-1 | King's Lynn |
| Saturday 16th December 2000 |
| Clevedon Town | 0-1 | Havant & Waterlooville |
| Crawley Town | 2-1 | Weymouth |
| Dorchester Town | 0-2 | Stafford Rangers |
| Folkestone Invicta | 1-0 | Tamworth |
| Halesowen Town | 1-1 | Merthyr Tydfil |
| King's Lynn | 1-4 | Margate |
| Moor Green | 1-2 | Burton Albion |
| Newport County | 3-0 | Cambridge City |
| Salisbury City | 3-0 | Fisher Athletic |
| Welling United | 1-0 | Bath City |
| Worcester City | 1-0 | Ilkeston Town |
| Saturday 23rd December 2000 |
| Bath City | 2-0 | Newport County |
| Cambridge City | 3-0 | Halesowen Town |
| Dorchester Town | 0-3 | Welling United |
| Fisher Athletic | 2-1 | Tamworth |
| Ilkeston Town | 2-1 | Havant & Waterlooville |
| Margate | 2-0 | Clevedon Town |
| Merthyr Tydfil | 1-5 | Burton Albion |
| Salisbury City | 0-0 | Folkestone Invicta |
| Stafford Rangers | 3-2 | Weymouth |
| Worcester City | 1-1 | King's Lynn |
| Tuesday 26th December 2000 |
| Burton Albion | 0-0 | Stafford Rangers |
| Clevedon Town | 0-1 | Bath City |
| Crawley Town | 0-0 | Margate |
| Folkestone Invicta | 2-1 | Fisher Athletic |
| Halesowen Town | 1-3 | Worcester City |
| Havant & Waterlooville | 3-1 | Salisbury City |
| King's Lynn | 2-2 | Ilkeston Town |
| Newport County | 2-0 | Merthyr Tydfil |
| Tamworth | 0-1 | Moor Green |
| Welling United | 0-2 | Cambridge City |
| Weymouth | 4-1 | Dorchester Town |
| Saturday 30th December 2000 |
| Welling United | 0-0 | Moor Green |
| Monday 1st January 2001 |
| Bath City | 0-0 | Weymouth |
| Dorchester Town | 0-1 | Havant & Waterlooville |
| Ilkeston Town | 2-1 | Burton Albion |
| Margate | 3-2 | Folkestone Invicta |
| Salisbury City | 1-1 | Crawley Town |
| Worcester City | 4-4 | Newport County |
| Saturday 6th January 2001 |
| Burton Albion | 2-1 | Newport County |
| Fisher Athletic | 1-1 | Clevedon Town |
| Folkestone Invicta | 0-2 | Bath City |
| Ilkeston Town | 2-1 | Merthyr Tydfil |
| King's Lynn | 2-0 | Crawley Town |
| Stafford Rangers | 3-2 | Havant & Waterlooville |
| Tamworth | 3-2 | Halesowen Town |
| Weymouth | 2-1 | Welling United |
| Worcester City | 0-1 | Margate |
| Tuesday 9th January 2001 |
| Fisher Athletic | 0-4 | Burton Albion |
| Saturday 13th January 2001 |
| Clevedon Town | 3-0 | Cambridge City |
| Dorchester Town | 2-1 | Salisbury City |
| Halesowen Town | 0-1 | Ilkeston Town |
| Merthyr Tydfil | 2-1 | Havant & Waterlooville |
| Moor Green | 1-0 | Worcester City |
| Welling United | 0-0 | Stafford Rangers |
| Tuesday 16th January 2001 |
| Salisbury City | 2-0 | Worcester City |
| Saturday 20th January 2001 |
| Bath City | 1-1 | Merthyr Tydfil |
| Clevedon Town | 1-0 | King's Lynn |
| Ilkeston Town | 0-1 | Welling United |
| Salisbury City | 4-1 | Stafford Rangers |
| Tamworth | 2-2 | Havant & Waterlooville |
| Weymouth | 1-1 | Burton Albion |
| Worcester City | 2-0 | Folkestone Invicta |
| Tuesday 23rd January 2001 |
| Burton Albion | 2-2 | Cambridge City |
| Saturday 27th January 2001 |
| Burton Albion | 2-2 | Bath City |
| Dorchester Town | 1-1 | Tamworth |
| Fisher Athletic | 1-0 | Worcester City |
| Folkestone Invicta | 2-1 | Halesowen Town |
| Havant & Waterlooville | 4-2 | Cambridge City |
| Ilkeston Town | 1-6 | Weymouth |
| Merthyr Tydfil | 0-2 | Margate |
| Moor Green | 0-2 | Salisbury City |
| Newport County | 5-2 | Clevedon Town |
| Stafford Rangers | 0-1 | King's Lynn |
| Welling United | 1-1 | Crawley Town |
| Monday 29th January 2001 |
| Worcester City | 1-2 | Dorchester Town |
| Tuesday 30th January 2001 |
| Tamworth | 3-1 | Bath City |
| Wednesday 31st January 2001 |
| Newport County | 0-4 | Fisher Athletic |
| Saturday 3rd February 2001 |
| Bath City | 1-1 | Ilkeston Town |
| Cambridge City | 0-3 | Fisher Athletic |
| Halesowen Town | 2-2 | Welling United |
| Moor Green | 1-1 | Merthyr Tydfil |
| Stafford Rangers | 1-0 | Dorchester Town |
| Tamworth | 4-1 | Salisbury City |
| Worcester City | 2-0 | Crawley Town |
| Saturday 10th February 2001 |
| Bath City | 4-0 | Halesowen Town |
| Cambridge City | 1-2 | Tamworth |
| Clevedon Town | 1-2 | Burton Albion |
| Crawley Town | 1-0 | Moor Green |
| Dorchester Town | 1-0 | Folkestone Invicta |
| Fisher Athletic | 1-1 | Ilkeston Town |
| Havant & Waterlooville | 5-1 | Weymouth |
| Merthyr Tydfil | 1-0 | King's Lynn |
| Salisbury City | 0-1 | Margate |
| Welling United | 0-1 | Worcester City |
| Tuesday 13th February 2001 |
| Burton Albion | 1-0 | Halesowen Town |
| Saturday 17th February 2001 |
| Clevedon Town | 2-3 | Newport County |
| Dorchester Town | 4-0 | Merthyr Tydfil |
| Folkestone Invicta | 1-2 | Weymouth |
| Halesowen Town | 3-0 | Salisbury City |
| Ilkeston Town | 1-1 | Crawley Town |
| King's Lynn | 0-0 | Worcester City |
| Moor Green | 0-0 | Cambridge City |
| Stafford Rangers | 6-2 | Bath City |
| Tamworth | 2-0 | Fisher Athletic |
| Welling United | 1-2 | Burton Albion |
| Tuesday 20th February 2001 |
| Burton Albion | 0-2 | Folkestone Invicta |
| Havant & Waterlooville | 0-0 | Halesowen Town |
| Ilkeston Town | 0-3 | Margate |
| Saturday 24th February 2001 |
| Bath City | 4-1 | Clevedon Town |
| Cambridge City | 0-0 | Ilkeston Town |
| Fisher Athletic | 2-3 | Stafford Rangers |
| Havant & Waterlooville | 2-1 | Dorchester Town |
| King's Lynn | 2-2 | Halesowen Town |
| Margate | 1-0 | Tamworth |
| Merthyr Tydfil | 1-0 | Salisbury City |
| Newport County | 5-0 | Folkestone Invicta |
| Tuesday 27th February 2001 |
| Clevedon Town | 0-1 | Worcester City |
| Fisher Athletic | 1-0 | Dorchester Town |
| Stafford Rangers | 1-0 | Merthyr Tydfil |
| Welling United | 2-1 | Havant & Waterlooville |
| Wednesday 28th February 2001 |
| Newport County | 3-0 | Bath City |
| Thursday 1st March 2001 |
| Weymouth | 1-0 | Crawley Town |
| Saturday 3rd March 2001 |
| Bath City | 2-2 | King's Lynn |
| Cambridge City | 1-1 | Stafford Rangers |
| Crawley Town | 1-0 | Clevedon Town |
| Fisher Athletic | 0-2 | Merthyr Tydfil |
| Ilkeston Town | 0-1 | Newport County |
| Margate | 1-0 | Halesowen Town |
| Moor Green | 1-1 | Welling United |
| Salisbury City | 1-3 | Tamworth |
| Worcester City | 1-1 | Burton Albion |
| Sunday 4th March 2001 |
| Dorchester Town | 0-1 | King's Lynn |
| Tuesday 6th March 2001 |
| Burton Albion | 2-1 | Moor Green |
| Fisher Athletic | 1-1 | Welling United |
| Havant & Waterlooville | 1-4 | Folkestone Invicta |
| Stafford Rangers | 1-1 | Tamworth |
| Wednesday 7th March 2001 |
| Newport County | 1-3 | Salisbury City |
| Saturday 10th March 2001 |
| Cambridge City | 2-0 | Moor Green |
| Clevedon Town | 8-1 | Fisher Athletic |
| Dorchester Town | 0-1 | Margate |
| Folkestone Invicta | 1-2 | Worcester City |
| Halesowen Town | 0-4 | King's Lynn |
| Ilkeston Town | 1-1 | Salisbury City |
| Newport County | 2-2 | Havant & Waterlooville |
| Stafford Rangers | 5-2 | Welling United |
| Tamworth | 4-0 | Merthyr Tydfil |
| Tuesday 13th March 2001 |
| Moor Green | 3-2 | Halesowen Town |
| Saturday 17th March 2001 |
| Bath City | 0-0 | Cambridge City |
| Ilkeston Town | 1-0 | Fisher Athletic |
| Margate | 1-1 | Salisbury City |
| Merthyr Tydfil | 2-0 | Folkestone Invicta |
| Tamworth | 2-2 | Dorchester Town |
| Welling United | 2-3 | Clevedon Town |
| Weymouth | 5-1 | Moor Green |
| Tuesday 20th March 2001 |
| Crawley Town | 4-3 | Fisher Athletic |
| Folkestone Invicta | 2-0 | Dorchester Town |
| Halesowen Town | 0-2 | Weymouth |
| King's Lynn | 2-2 | Havant & Waterlooville |
| Moor Green | 0-4 | Margate |
| Friday 23rd March 2001 |
| Worcester City | 2-0 | Cambridge City |
| Saturday 24th March 2001 |
| Dorchester Town | 1-2 | Halesowen Town |
| Folkestone Invicta | 0-2 | Burton Albion |
| King's Lynn | 2-0 | Welling United |
| Margate | 2-0 | Bath City |
| Merthyr Tydfil | 0-0 | Tamworth |
| Newport County | 0-0 | Weymouth |
| Stafford Rangers | 0-2 | Moor Green |
| Monday 26th March 2001 |
| Cambridge City | 1-2 | King's Lynn |
| Newport County | 1-0 | Stafford Rangers |
| Tuesday 27th March 2001 |
| Bath City | 3-0 | Dorchester Town |
| Weymouth | 3-0 | Fisher Athletic |
| Thursday 29th March 2001 |
| Worcester City | 1-0 | Havant & Waterlooville |
| Saturday 31st March 2001 |
| Bath City | 2-1 | Tamworth |
| Burton Albion | 2-1 | Crawley Town |
| Cambridge City | 2-0 | Clevedon Town |
| Fisher Athletic | 3-0 | Moor Green |
| Folkestone Invicta | 1-2 | King's Lynn |
| Havant & Waterlooville | 2-0 | Stafford Rangers |
| Ilkeston Town | 1-0 | Dorchester Town |
| Merthyr Tydfil | 0-0 | Halesowen Town |
| Newport County | 0-0 | Margate |
| Welling United | 2-1 | Salisbury City |
| Weymouth | 4-0 | Worcester City |
| Tuesday 3rd April 2001 |
| Dorchester Town | 0-3 | Moor Green |
| Havant & Waterlooville | 2-0 | Bath City |
| Merthyr Tydfil | 2-0 | Clevedon Town |
| Stafford Rangers | 1-1 | Cambridge City |
| Weymouth | 3-1 | Salisbury City |
| Wednesday 4th April 2001 |
| Newport County | 3-1 | Ilkeston Town |
| Saturday 7th April 2001 |
| Clevedon Town | 0-1 | Folkestone Invicta |
| Crawley Town | 1-2 | Bath City |
| Dorchester Town | 1-0 | Cambridge City |
| King's Lynn | 1-0 | Merthyr Tydfil |
| Margate | 2-0 | Ilkeston Town |
| Moor Green | 1-1 | Weymouth |
| Salisbury City | 1-0 | Burton Albion |
| Stafford Rangers | 3-0 | Fisher Athletic |
| Tamworth | 1-0 | Newport County |
| Worcester City | 1-2 | Welling United |
| Tuesday 10th April 2001 |
| Crawley Town | 2-1 | Halesowen Town |
| Fisher Athletic | 1-0 | Bath City |
| Havant & Waterlooville | 1-0 | Merthyr Tydfil |
| Weymouth | 1-0 | Margate |
| Thursday 12th April 2001 |
| Crawley Town | 1-1 | Ilkeston Town |
| Havant & Waterlooville | 1-1 | Burton Albion |
| Friday 13th April 2001 |
| Newport County | 0-1 | Worcester City |
| Saturday 14th April 2001 |
| Burton Albion | 1-0 | Ilkeston Town |
| Clevedon Town | 2-2 | Merthyr Tydfil |
| Crawley Town | 2-0 | Havant & Waterlooville |
| Folkestone Invicta | 2-2 | Margate |
| Halesowen Town | 0-2 | Moor Green |
| King's Lynn | 0-4 | Cambridge City |
| Salisbury City | 0-2 | Dorchester Town |
| Tamworth | 2-0 | Stafford Rangers |
| Welling United | 4-0 | Fisher Athletic |
| Weymouth | 1-1 | Bath City |
| Monday 16th April 2001 |
| Bath City | 0-1 | Salisbury City |
| Cambridge City | 4-1 | Welling United |
| Dorchester Town | 1-1 | Weymouth |
| Fisher Athletic | 1-2 | Folkestone Invicta |
| Havant & Waterlooville | 1-0 | Clevedon Town |
| Ilkeston Town | 1-1 | King's Lynn |
| Margate | 3-0 | Crawley Town |
| Merthyr Tydfil | 2-1 | Newport County |
| Moor Green | 0-1 | Tamworth |
| Stafford Rangers | 0-2 | Burton Albion |
| Worcester City | 4-1 | Halesowen Town |
| Wednesday 18th April 2001 |
| Fisher Athletic | 0-3 | Havant & Waterlooville |
| Saturday 21st April 2001 |
| Burton Albion | 4-0 | Havant & Waterlooville |
| Clevedon Town | 1-2 | Margate |
| Crawley Town | 0-2 | Tamworth |
| Folkestone Invicta | 3-1 | Merthyr Tydfil |
| Halesowen Town | 3-0 | Cambridge City |
| King's Lynn | 2-0 | Dorchester Town |
| Moor Green | 3-2 | Bath City |
| Salisbury City | 2-0 | Ilkeston Town |
| Welling United | 3-1 | Newport County |
| Weymouth | 0-2 | Stafford Rangers |
| Worcester City | 1-2 | Fisher Athletic |
| Sunday 22nd April 2001 |
| Havant & Waterlooville | 0-0 | Crawley Town |
| Tuesday 24th April 2001 |
| Burton Albion | 1-0 | Weymouth |
| Clevedon Town | 2-1 | Stafford Rangers |
| Fisher Athletic | 1-3 | Newport County |
| Folkestone Invicta | 2-1 | Crawley Town |
| Havant & Waterlooville | 0-1 | Ilkeston Town |
| Margate | 3-2 | Dorchester Town |
| Salisbury City | 1-4 | King's Lynn |
| Thursday 26th April 2001 |
| Cambridge City | 0-2 | Margate |
| Halesowen Town | 2-2 | Havant & Waterlooville |
| Saturday 28th April 2001 |
| Bath City | 0-2 | Welling United |
| Cambridge City | 2-2 | Weymouth |
| Dorchester Town | 1-3 | Burton Albion |
| Fisher Athletic | 0-0 | Halesowen Town |
| Havant & Waterlooville | 0-0 | Moor Green |
| Ilkeston Town | 3-0 | Folkestone Invicta |
| Margate | 3-0 | Worcester City |
| Merthyr Tydfil | 0-2 | Crawley Town |
| Newport County | 1-3 | King's Lynn |
| Stafford Rangers | 4-2 | Salisbury City |
| Tamworth | 0-2 | Clevedon Town |
| Monday 30th April 2001 |
| Cambridge City | 0-1 | Dorchester Town |
| Tuesday 1st May 2001 |
| Crawley Town | 2-2 | Burton Albion |
| Margate | 0-0 | Newport County |
| Weymouth | 0-1 | Clevedon Town |
| Thursday 3rd May 2001 |
| Crawley Town | 1-0 | Worcester City |
| Salisbury City | 0-0 | Havant & Waterlooville |
| Saturday 5th May 2001 |
| Burton Albion | 2-0 | Margate |
| Clevedon Town | 2-3 | Ilkeston Town |
| Crawley Town | 1-2 | Newport County |
| Folkestone Invicta | 0-3 | Stafford Rangers |
| Halesowen Town | 2-0 | Bath City |
| Havant & Waterlooville | 2-0 | Worcester City |
| King's Lynn | 0-1 | Fisher Athletic |
| Moor Green | 3-1 | Dorchester Town |
| Salisbury City | 2-1 | Cambridge City |
| Welling United | 1-0 | Merthyr Tydfil |
| Weymouth | 0-2 | Tamworth |
|